Thylakoid targeting of Tat passenger proteins shows no DeltapH dependence in vivo
نویسندگان
چکیده
منابع مشابه
Functional assembly of thylakoid deltapH-dependent/Tat protein transport pathway components in vitro.
Assembly of the components of the thylakoid deltapH-dependent/Tat protein transport machinery was analyzed in vitro. Upon incubation with intact chloroplasts, precursors to all three components, Hcf106, cpTatC and Tha4, were imported into the organelle and assembled into characteristic endogenous complexes. In particular, all of the imported cpTatC and approximately two-thirds of the imported H...
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The thylakoid transmembrane DeltapH is the sole energy source driving translocation of precursor proteins by the DeltapH/Tat machinery. Consequently, proton translocation must be coupled to precursor translocation. For the precursor of the 17 kDa protein of the oxygen-evolving complex (pOE17), the protein translocation process is characterized by a steep drop in efficiency at an external pH bel...
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Among the protein translocation pathways of the thylakoid membrane in chloroplasts, the DeltapH/TAT pathway is unique in several aspects. In vitro transport assays with isolated chloroplasts or thylakoids have defined the trans-thylakoidal proton gradient as the sole requirement for effecting transport. From these studies, evidence has also accumulated indicating that, in contrast to the remain...
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In vitro import assays have shown that the thylakoid twin-arginine translocase (Tat) system transports folded proteins in a unidirectional manner. Here, we expressed a natural substrate, pre-23K, and a 23K presequence-green fluorescent protein (GFP) chimera in vivo in tobacco protoplasts. Both are imported into chloroplasts, targeted to the thylakoids, and processed to the mature size by the lu...
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D espite their name, the passenger proteins aren't just along for the ride during mitosis. They are busy helping control the attachment of spindle fi bers to the chromosomes and ensuring that the cell splits after the chromosomes part. INCENPs' many locations But it was the proteins' seemingly bizarre movements that fi rst caught the eye of William Earnshaw (now at the University of Edinburgh i...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2003
ISSN: 1460-2075
DOI: 10.1093/emboj/cdg081